文献：Indocyanine Green-Loaded Silver Nanoparticle@PolyanilineCore/Shell Theranostic Nanocomposites for Photoacoustic/Near-Infrared Fluorescence Imaging-Guided and SingleLight Triggered Photothermal and Photodynamic Therapy

文献链接：

https://www.nstl.gov.cn/paper_detail.html?id=1451f299ed14570ca081de73158d6d18

作者：Xiaoxiao Tan, Jinping Wang, Xiaojuan Pang, Li Liu, Qi Sun, Qing You, Fengping Tan, and Nan Li

相关产品：NH2-PEG2000（氨基-聚乙二醇2000）

原文摘要：

Photoacoustic (PA)/near-infrared fluorescence (NIRF) dual-modal imaging-guided phototherapy has been wide explored very recently. However, the development of high-efficiency and simplified-performed theranostic system for amplifying imaging guided photothermal therapy/photodynamic therapy (PTT/PDT) is still a great challenge. Herein, a single-light triggered indocyanine green (ICG)-loaded PEGylation silver nanoparticle core/polyaniline shell (Ag@PANI) nanocomposites (ICG-Ag@PANI) for PA/NIRF imaging guided enhanced PTT/PDT synergistic effect has been successfully constructed. In this study, the synthesized Ag@PANI nanocomposites are not only utilized as the promising photothermal agent, but also as the potential nanovehicles for loading photosensitizer ICG via π-π stacking and hydrophobic interaction. The as-prepared ICG-Ag@PANI possesses many superior properties such as strong optical absorption in near-infrared (NIR) region, enhanced photo-stability of ICG as well as outstanding NIR laser-induced local hyperthermia and reactive oxygen species (ROS) generation. In the in vivo study, PA/NIRF dual-modal imaging confirms the accumulation and distribution of ICG-Ag@PANI in tumor region via enhanced permeability and retention (EPR) effect. Moreover, the PTT effect of ICG-Ag@PANI rapidly raised the tumor temperature to 56.8 °C within 5 min. It is also demonstrated that the cytotoxic ROS generation ability of ICG is well maintained after being loaded onto Ag@PANI nanocomposites. Remarkably, in comparison with PTT or PDT alone, the single 808 nm NIR laser-triggered combined PTT/PDT therapy exhibits the enhanced HeLa cells lethality in vitro and tumor growth inhibition in vivo.

NH2- PEG2000 是一种聚乙二醇（PEG）衍生物，其中 “2000” 表示其分子量约为 2000。分子结构的一端是氨基（- NH₂），另一端是聚乙二醇链的末端。聚乙二醇链由多个乙二醇单元（- CH₂CH₂O -）重复组成，其主链具有良好的柔韧性。氨基官能团使得该化合物能够参与多种化学反应，是一种比较重要的活性基团。基于NH2- PEG2000的相关特性，该文献介绍ICG-Ag@PANI合成流程如下：

图：合成流程

将制备的AgNPs溶液离心，仔细取上清。将分离后的AgNPs加入苯胺和SDS的混合溶液中，然后超声处理。立即将过硫酸铵溶液注入AgNPs/苯胺/SDS混合溶液中，然后涡旋。将最终溶液再搅拌，以进行完全掺杂和聚合。由此得到了含Ag核和PANI壳（Ag-PANI）的纳米复合材料。聚乙二醇化方面，将原始Ag-PANI与NH2-PEG2000混合，搅拌。最终，将树脂树脂溶液离心，去除多余的PANI和其他反应物，并在PBS中重新分散。PANI的合成按照与Ag@PANI相同的方法进行，没有在苯胺/SDS混合溶液中加入AgNPs。将Ag@PANI溶液与不同浓度的ICG混合，过夜搅拌。为了纯化，多余的ICG在12-14 KD截止膜中透析去除。收集到的ICG-Ag@PANI在低温黑暗中保存。

图：吸收光谱图像

结论：

该文献成功合成了单光触发聚氰胺绿（ICG）聚乙二醇银纳米颗粒核/聚苯胺壳（Ag@PANI）纳米复合材料（ICG-Ag@PANI）。该产品具有许多良好的特性：近红外（NIR）区域的强光吸收，增强ICG的光稳定性，以及出色的NIR激光诱导局部热疗和活性氧（ROS）生成等。数据表明，将ICG装入Ag@PANI纳米复合材料后，其细胞poison性ROS的生成能力得到了良好的维持。