文献：

Effect of vacuum impregnation of red sea bream (Pagrosomus major) with herring AFP combined with CS@Fe3O4 nanoparticles during freeze-thaw cycles

文献链接：

作者：

Linyu Nian, Ailing Cao, Luyun Cai, Hongwu Ji, Shucheng Liu

相关产品：

CS@Fe3O4 nanoparticles

原文摘要：

   This study examined the effect of herring antifreeze protein (hAFP) combined with chitosan magnetic nanoparticles (CS@Fe3O4), applied through vacuum impregnation, on protein conformation and moisture state of red sea bream during freeze-thaw cycles. DSC and dynamic rheology were used to analyze physicochemical changes. Raman, intrinsic fluorescence and second derivative UV spectrometry were used to explore the structural changes of myofibrillar proteins. Surface hydrophobicity, particle size and zeta-potential were used to analyze protein aggregation and denaturation. AV, POV, and TBA were used to determine the degree of lipid oxidation. LF-NMR and MRI were used to test water migration and distribution. The results indicated that pretreatment of red sea bream through vacuum impregnation with hAFP combined with CS@Fe3O4 solutions could minimize freeze-thaw damage, prevent protein and lipid oxidation. Therefore, these results confirm the importance of hAFP as a valuable food additive in frozen foods.

“CS@Fe3O4 nanoparticles” 指的是壳聚糖（Chitosan，CS）包覆四氧化三铁（Fe3O4）纳米粒子。四氧化三铁纳米粒子（Fe3O4 nanoparticles）是一种磁性纳米材料，其晶体结构属于反尖晶石结构，化学组成为 Fe3O4₄，实际上是由 FeO 和 Fe2O3组成的复合物（FeO・Fe2O3）。它具有超顺磁性，在外界磁场作用下能够快速磁化，而当磁场移除后，磁性又能很快消失。这种特性使得 Fe3O4纳米粒子在磁性分离、靶向化合物递送等众多领域有应用。壳聚糖（CS）是一种天然多糖，它是由自然界存在的几丁质经过脱乙酰作用得到的。壳聚糖分子中含有大量的氨基（- NH₂）和羟基（- OH），这些官能团使得壳聚糖具有良好的生物相容性、可生物降解性和吸附性等诸多优良性质。用壳聚糖包覆 Fe3O4纳米粒子，可以改善纳米粒子的稳定性、分散性和生物相容性等性能。基于CS@Fe3O4 nanoparticles的特性，该文献实验流程如下：

图：制备流程

将红鲷肉切成小块，在一定浓度的氯化钠溶液，含海藻糖、CS@Fe3O4纳米颗粒保存，然后在低温下保存，然后再解冻。F-T过程重复多次，分别命名。未经真空浸渍的样品在低温冷冻保存，再解冻，冻融循环后分别命名。

图：对照图像

结论：

该文献实验结果表明，用hAFP联合CS@Fe3O4溶液真空浸渍预处理红鲷，可减少冻融损伤，防止蛋白质和脂质氧化。hAFP因其具有降低冰点、修饰冰晶形态、抑制重结晶及提高生物抗冻能力等特性，在与CS@Fe3O4纳米结合后，表现出良好的效果。