文献：Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=5cab201bf401943b3fa7f55171930b03&site=xueshu_se

作者：Jing Tan , Cuizhu Wang , Hailin Zhu , Baisong Zhou , Lingxin Xiong , Fang Wang , Pingya Li  and Jinping Liu

相关产品：

5-HETE, 16(R)-HETE , 19(S)-HETE

原文摘要：Blood stasis syndrome (BSS) is one of the most common Chinese medicine patterns in coronary heart disease. Our previous work proved that Xueshuan Xinmaining Tablet (XXT) could treat blood stasis through regulating the expression of F13a1, Car1 and Tbxa2r. In the current study, the effect and mechanism of XXT on BSS was comprehensively and holistically investigated based on a metabolomics approach. Urine and plasma samples of 10 BBS rats treated with XXT (XT), 9 BSS model rats (BM) and 11 normal control (NC) rats were collected and then determined by UPLC-Q/TOP-MS. Multivariate analyses were applied to distinguish differentiate urinary and plasma metabolite patterns between three groups. Results showed that a clear separation of three groups was achieved. XT group was located between BM group and NC group, and showing a tendency of recovering to NC group, which was consistent with the results of hemorheological studies. Some significantly changed metabolites like cortexolone, 3α,21-dihydroxy-5β-pregnane-11,20-dione and 19S-hete and leukotriene A4, chiefly involved in steroid hormone biosynthesis, arachidonic acid metabolism and lipid metabolism, were found and identified to explain the mechanism. These potential markers and their corresponding pathways will help explain the mechanism of BSS and XXT treatment. This work also proves that metabolomics is effective in traditional Chinese medicinal research.

5-HETE（(±)-5-HETE）是一种脂肪酸，是花生四烯酸的氧化衍生物，同时也是5(S)-HETE和5(R)-HETE的混合物。16(R)-HETE是花生四烯酸盐级联的细胞色素P450分支的代谢产物。19(S)-HETE是一种由细胞色素P450酶产生的花生四烯酸代谢物。5-HETE、16(R)-HETE和19(S)-HETE作为花生四烯酸的代谢产物，在生物体内发挥着重要的调节作用。它们各自具有生物活性，并在一些方面发挥着重要作用。这些代谢产物的研究具有重要意义。

图为：5-HETE结构式

5-HETE 、16(R)-HETE、19(S)-HETE在bss相关代谢物实验中的相关应用：

收集经XXT（XT）处理的BBS大鼠、BSS模型大鼠（BM）和正常对照（NC）大鼠的尿液和血浆样本，然后采用UPLC-Q/TOP-MS检测。采用多变量分析来区分三组间的尿和血浆代谢物模式。XT组位于BM组与NC组之间，向NC组有恢复的趋势，这与血液流变学研究结果一致。皮质醇酮、3α、21-二羟基-5β-孕酮-11、11、19S-hete和白三烯A4等代谢物，主要参与类固醇激素生物合成、花生四烯酸代谢和脂质代谢产物。

图为：不同组差异代谢物的归一化强度

信息总结可以看出，5-HETE（羟基二十碳四烯酸）、17-羟基孕酮、16（R）-HETE数值降低，5-HETE、16(R)-hETE和19(S)-hETE水平升高，表明BSS导致花生四烯酸代谢紊乱，从而可能降低BSS大鼠较高的血液流变学指标。潜在的标记物及其相应的通路将有助于解释BSS和XXTTreatment 的机制，代谢组学在中药研究中是有效的。