2025-04-02 作者：wff 来源：文献：
A chitosan-based multifunctional hydrogel containing in situ rapidly bioreduced silver nanoparticles for accelerating infected wound healing
文献链接：
https://pubs.rsc.org/en/content/articlelanding/2022/tb/d1tb02850b
作者：
Fengxin Zhao,Yifan Liu,Tao Song,Bin Zhang,Dongxiao Li,Yumei Xiao and Xingdong Zhang
相关产品：
季铵盐-单宁酸-壳聚糖（QATAC）
原文摘要：
Generally, bacterial infection seriously hinders the wound healing process, so it is crucial to safeguard the wound from severe infection. Besides, multifunctional hydrogel dressings (self-healing, injectable, antibacterial and adaptable) seem to be conducive to meet the needs of wound healing. Here, a double-crosslinked multifunctional hydrogel (COC hydrogel) based on quaternized chitosan, methacrylate anhydride-modified collagen and oxidized dextran was developed. The double-crosslinked network improved the stability of the hydrogel while not destroying the functionality of the Schiff base bond. More importantly, silver ions were rapidly in situ bioreduced to silver nanoparticles (AgNPs) during the formation of the COC hydrogel, which can essentially avoid the dispersion and agglomeration problems. The obtained COC@AgNP hydrogel had good biocompatibility compared with that loaded with silver ions and excellent antibacterial properties compared with that loaded with the same amount of commercial AgNPs. In vivo results indicated that the COC@AgNP hydrogel accelerated the healing process of infected full-thickness skin defects through anti-infection, anti-inflammation, stimulating collagen deposition, and promoting the formation of epithelia and blood vessels. Collectively, the COC@AgNP hydrogel has good potential for clinical infected wound dressing applications.
本文献创新构建了一种多功能COC复合水凝胶敷料体系，通过分子工程策略实现多重生物学功能的集成。该水凝胶由季铵化壳聚糖、甲基丙烯酸酐修饰胶原蛋白和氧化葡聚糖共混形成互穿双网络结构，其中离子交联与化学交联协同作用增强了凝胶稳定性，同时保留动态希夫碱键的响应性特征。
在凝胶化过程中，通过原位生物还原反应实现银离子到纳米银（AgNP）的瞬时转化，这种"一步法"合成策略有效克服了传统纳米银分散性差。对比实验显示，该COC@AgNP水凝胶较单纯载银离子体系表现出更优的生物相容性。
动物实验进一步证实，该智能敷料通过上调胶原蛋白I/III型表达比例，促进肉芽组织有序重塑，加速慢性创面愈合进程。其作用机制可能与凝胶基质提供的湿润微环境、持续释放的银离子抗菌效应以及动态交联网络对细胞迁移的引导作协同相关。这种兼具结构稳定性与生物活性的设计策略，为复杂伤口管理提供了具有转化潜力的新型解决方案。
