文献：

Study of Projections From the Entopeduncular Nucleus to the Thalamus of the Rat，THE JOURNAL OF COMPARATIVE NEUROLOGY 426:366–377 (2000).

文献链接：

https://pubmed.ncbi.nlm.nih.gov/10992243/

作者：

H T Kha, D I Finkelstein, D V Pow, A J Lawrence, M K Horne

相关产品：

生物素-葡聚糖 DEX-BIOTIN

原文摘要：

The entopeduncular nucleus (EP) is a major outflow nucleus of the basal ganglia and innervates the lateral habenula, parafascicular, pedunculopontine, ventrolateral (VL), ventromedial (VM), and mediodorsal thalamic nuclei. This study investigated the morphology of single axons of entopeduncular neurons projecting to the motor thalamus by placing small injections of dextran biotin into the EP and reconstructing drawings of single axons from serial sections. There were two populations of entopeduncular-thalamic projection axons: those that projected only to the motor thalamus (VL and VM) and those that projected to both the motor thalamus and other nuclei (e.g., the habenula). The neurochemistry of EP neurons projecting to the thalamus was investigated by injecting the retrograde tracer FluoroGold into the VL and VM thalamic nuclei to retrogradely fill entopeduncular projection neurons. These were subsequently immunohistochemically labeled for choline acetyl transferase, gamma-aminobutyric acid (GABA), and glutamate. Consistent with previous studies, significant proportions of these neurons were GABA immunoreactive. In addition, approximately half of the entopeduncular-thalamic projecting neurons were found to be cholinergic. This excitatory input is most likely derived from axons that branch as they pass through the motor thalamus to the lateral habenula.

（EP）是基底节的主要流出核，支配外侧缰核、束旁核、足桥核、腹外侧核（VL）、腹内侧核（VM）和丘脑背中核。通过在EP中少量注射葡聚糖生物素并从连续切片中重建单个轴突的图形，研究了向运动丘脑投射的足内神经元的单个轴突的形态。有两类内足丘脑投射轴突：一类仅投射到运动丘脑（VL和VM），另一类同时投射到运动下丘脑和其他细胞核（如缰核）。通过向VL和VM丘脑核注射逆行示踪剂FluoroGold以逆行填充足内投射神经元，研究了投射到丘脑的EP神经元的神经化学。随后对其进行胆碱乙酰转移酶、γ-氨基丁酸（GABA）和谷氨酸的免疫组织化学标记。

应用说明：生物素葡聚糖用于组织染色，以制备神经元，用于通过抗生物素缀合的检测系统进行检测。它被神经元过程吸收并双向转运。顺行方向浸润轴突突，逆行方向浸润树突突。 将生物素葡聚糖注射到大脑或脊髓后两天至两周，可以在固定和切片的组织中观察到染色。它也可以用于切割神经束。在与抗生物素-辣根过氧化物酶缀合物和电子致密底物3，3′-二氨基联苯胺（DAB）孵育后，通过光学或电子显微镜检测生物素化神经元。