文献：

Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells

文献链接：

https://pubmed.ncbi.nlm.nih.gov/31545500/

作者：

Xiaoyang Hou 1, Yanyu Pang 1, Xinxin Li 1, Chunsheng Yang 2, Wenlou Liu 1, Guan Jiang 1, Yanqun Liu 1

原文摘要：

A novel core-shell type thermo-nanoparticle (CSTNP) coloaded with temozolomide (TMZ) and the fluorescein new indocyanine green dye IR820 (termed IT-CSTNPs) was designed and combined with a near-infrared (NIR) laser to realize its photothermal conversion. The IT-CSTNPs were prepared using a twostep synthesis method and comprised a thermosensitive shell and a biodegradable core. IR820 and TMZ were entrapped in the shell and the core, respectively. Dynamic light scattering results demonstrated that the average hydrodynamic size of the IT-CSTNPs was 196.4±3.1 nm with a ζ potential of -24.9±1.3 mV. The encapsulation efficiencies of TMZ and IR820 were 6.1 and 16.6%, respectively. Temperature increase curves under NIR laser irradiation indicated that the IT-CSTNPs exhibited the desired photothermal conversion efficiency. The in vitro drug release curves revealed a suitable release capability of ITCSTNP under physiological conditions, whereas NIR laser irradiation accelerated the drug release. Inverted fluorescence microscopy and flow cytometry results revealed that the uptake of ITCSTNPs by A375 melanoma cells occurred in a concentrationdependent manner. Confocal laser scanning microscopy results indicated that ITCSTNPs entered tumour cells via endocytosis and were located in intercellular lysosomes. In summary, the present study explored the photothermal conversion capability, cellular uptake, and intracellular localization of IT-CSTNPs.

PLGA是一种可降解聚合物，可以快速从体内去除，是一种药物传递纳米材料。在核心壳脂质纳米颗粒中,PLGA通常作为核心材料,而脂质则作为外壳。这种结构的设计可以结合PLGA的生物降解性和脂质的生物相容性,提供一种药物递送方式。

图为：IGU-PLGA-NPs纳米粒子及其制备方法和应用与流程

IT-CSTNPs的制备方法。首先，将TMZ溶解在盐酸中，形成水相。然后，将的PLGA溶解在的二氯甲烷（DCM）中，形成油相。将水相加入到油相中，用超声波处理器将得到的混合物乳化，形成初始乳化液。然后将乳液滴入PVA溶液中，再次乳化。将均匀乳液在室温下搅拌过夜，使DCM变性。PLGA-TMZ纳米颗粒离心，用超纯水洗涤三次以供后续使用。

图为：PLGA壳聚糖纳米颗粒控释递送系统的制备、表征

结论：PLGA因其良好的生物降解性和生物相容性而常被用作聚合物核心。DPPC作为脂质壳包裹PLGA，形成细胞状结构。简单的PLGA纳米颗粒很容易通过调理从血液循环中去除，暴露的PLGA在进入细胞时遇到困难。脂质层可以作为一个分子栅栏来保护PLGA核心，并提高tumour细胞对纳米颗粒的吸收。