2025-03-17 作者：wff 来源：文献：
One-step facile synthesis of hyaluronic acid functionalized fluorescent gold nanoprobes sensitive to hyaluronidase in urine specimen from bladder cancer patients,Talanta,Volume 130,2014,Pages 408-414,ISSN 0039-9140,
文献链接：
https://pubmed.ncbi.nlm.nih.gov/25159428/
作者：
Dan Cheng, Weiye Han, Kuncheng Yang, Yang Song, Mingdong Jiang, Erqun Song,
相关产品：
文献摘要：
Gold nanoparticles (AuNPs) have been widely used to develop fluorescence resonance energy transfer (FRET) sensors to detect biological substances, environmental pollutants, and disease markers due to their superior quenching capacity to fluorescence signals. In this study, we report the one-step facile synthesis of fluorescein isothiocyanate-labeled hyaluronic acid (FITC-HA) functionalized fluorescent AuNPs based FRET nanoprobes (FITC-HA-AuNPs) via chemical reduction of HAuCl4 by using FITC-HA as both a reducing and stabilizing agent. Then the FITC-HA-AuNPs FRET nanoprobes were used to detect hyaluronidase (HAase), a new type of disease marker, based on the specific enzymatic degradation of HAase to HA. Compared with similar work, the FITC-HA-AuNPs nanoprobes were much easier to prepare and the detection sensitivity was also high for HAase to reach a detection limit of 0.63 U mL(-1). More importantly, they also allowed for rapid HAase detection (within 3h) even in complex biological specimens (urine specimens from patients with bladder cancer) with satisfactory accuracy (recovery efficiency in the range of 92.8-106.9% with RSD ≤ 4.85%). Our studies suggested that such a novel design of FITC-HA-AuNPs FRET nanoprobes developed for sensitive, rapid and accurate detection of HAase had exciting potentials for clinical diagnosis of HAase-related diseases, such as bladder cancer.
金纳米粒子（AuNPs）由于其对荧光信号的猝灭能力，已被Wide用于开发荧光共振能量转移（FRET）传感器，以检测生物物质、环境污染物。使用FITC-HA-AuNPs FRET纳米探针检测新型疾病标志物透明质酸蛋白酶（HAase）。与类似的工作相比，FITC-HA-AuNPs纳米探针更容易制备，并且HAase的检测灵敏度也很高，达到0.63 U mL（-1）的检测极限。这种FITC-HA-AuNPs FRET纳米探针用于敏感、快速和准确地检测HAase。
荧光素-透明质酸 FITC-HA在一步简易合成对膀胱Cancer症患者尿液标本中透明质酸酶敏感的透明质酸功能化荧光金纳米探针的应用
图为：荧光素-透明质酸结构式
应用方法：FITC–HA–AuNPs是根据我们之前的工作合成的，并进行了一些修改[41]。在典型的合成程序中，在搅拌下，将2.00 mg（2.5 nmol）的FITC–HA溶解在瓶中的1.5 mL 0.01 mol L 1磷酸盐缓冲溶液（NaH2PO4，Na2HPO4，pH 7.4）（PB）中，并将混合物缓慢加热至60 1C。然后，向上述溶液中滴加50μL 1%（W/V）HAuCl4水溶液。将溶液逐渐加热至80℃，直到溶液颜色变为粉红色。冷却至室温后，将产品储存在4℃的黑暗中。
