文献：

Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability.

文献链接：

https://www.docin.com/p-2103732743.html

相关产品：

荧光素-葡聚糖 FITC-Dextran

原文摘要：

The blood-brain barrier (BBB) is formed in part by vascular endothelial cells that constitute thecapillaries and microvessels of the brain, The function of this barrier is to maintain homeostasiswithin the brain microenvironment and buffer the brain from changes in the periphery. Adysfunction of the BBB would permit circulating molecules and pathogens typically restricted tothe periphery to enter the brain and interfere with normal brain function, As increasedpermeability of the BBB is associated with several neuropathologies, it is important to have areliable and sensitive method that determines BBB permeability and the degree of BBB disruptionA detailed protocol is presented for assessing the integrity of the BBB by transcardial perfusion ofa 10000 Da FITC labeled dextran molecule and its visualization to determine the degree ofextravasation from brain microvessels.

血脑屏障（BBB）部分由构成大脑毛细Blood vessels 和微Blood vessels 的Blood vessels 内皮细胞形成。该屏障的功能是维持大脑微环境内的稳态，并缓冲大脑免受外围变化的影响。血脑屏障的功能紊乱会使循环分子和病原体（通常局限于外周）进入大脑并干扰正常的大脑功能。提出通过对FITC标记的葡聚糖分子进行心内灌注及其可视化来评估血脑屏障完整性，以确定脑微Blood vessels 的工作程度。FITC标记葡聚糖的好处是：由于荧光检测的高灵敏度及其与血浆蛋白的可忽略不计的结合，可以测量低脑内荧光素浓度。

FITC葡聚糖，会充满脑毛细Blood vessels ，并可使用荧光显微镜或在488激光的激发下观察。然后可以用FlTC葡聚糖对脑毛细Blood vessels 和周围薄壁组织进行成像和分析，以评估血脑屏障的完整性。

异硫氰酸荧光素（FITC）标记的葡聚糖。FITC-葡聚糖是由不同长度的支链葡萄糖分子组成的多糖。可用于根据所用葡聚糖的大小确定BBB的溶质、离子和蛋白质渗透性。因为FITC葡聚糖是外源性的，可以以离散的间隔珠化，与lgG等内源性分子相比，它们可以提供关于BBB渗透的时间过程和持续时间的有价值的信息。