文献：Biomimetic nanotherapy: core–shell structured nanocomplexes based on the neutrophil membrane for targeted therapy of lymphoma

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=106a04n0s57g0me0e61a00c0wq632866&site=xueshu_se

作者：Qiangqiang Zhao, Duanfeng Jiang , Xiaoying Sun, Qiuyu Mo, Shaobin Chen , Wansong Chen , Rong Gui and Xianjun Ma

相关产品：DSPE-PEG-FITC

原文摘要：Background: Non-Hodgkin’s lymphoma (NHL) is a malignant disease of lymphoid tissue. At present, chemotherapy is still the main method for the treatment of NHL. R-CHOP can signifcantly improve the survival rate of patients. Unfortunately, DOX is the main cytotoxic drug in R-CHOP and it can lead to adverse reactions. Therefore, it is particularly important to uncover new treatment options for NHL.

Results: In this study, a novel anti-tumor nanoparticle complex Nm@MSNs-DOX/SM was designed and constructed in this study. Mesoporous silica nanoparticles (MSNs) loaded with Doxorubicin (DOX) and anti-infammatory drugs Shanzhiside methylester (SM) were used as the core of nanoparticles. Neutrophil membrane (Nm) can be coated with multiple nanonuclei as a shell. DOX combined with SM can enhance the anti-tumor efect, and induce apoptosis of

lymphoma cells and inhibit the expression of infammatory factors related to tumorigenesis depending on the regulation of Bcl-2 family-mediated mitochondrial pathways, such as TNF-α and IL-1β. Consequently, the tumor microenvironment (TME) was reshaped, and the anti-tumor efect of DOX was amplifed. Besides, Nm has good biocompatibility and can enhance the EPR efect of Nm@MSNs-DOX/SM and increase the efect of active targeting tumors.

Conclusions: This suggests that the Nm-modifed drug delivery system Nm@MSNs-DOX/SM is a promising targeted chemotherapy and anti-infammatory therapy nanocomplex, and may be employed as a specifc and efcient antiLymphoma therapy.

DSPE-PEG-FITC是由1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺（DSPE）、聚乙二醇（PEG）和异硫氰酸荧光素（FITC）组成。DSPE作为一种磷脂成分，赋予了分子良好的脂质体形成能力和生物相容性，使其能够轻易地与细胞膜等生物膜结构相互作用。PEG链段则具有亲水性和空间位阻效应，一方面增加了整个分子在水溶液中的溶解性和稳定性，另一方面可以减少非特异性蛋白吸附，延长在体内的循环时间。FITC作为荧光标记部分，应用于生物成像细胞标记等众多生物领域。可以用来标记Nm vesicles。

DSPE-PEG-FITC标记Nm泡

CD47是一种在细胞表面上表达“自我识别”的分子标记物，通过与嗜中性粒细胞表面表达的受体（信号调节蛋白α，SIRPα）结合，向下游发送免疫抑制信号。Ly6c是一种14kd的蛋白，在中性粒细胞、单核细胞和树突状细胞中表达，其中中性粒细胞是突出的。此外，它是一种中性粒细胞特异性膜蛋白，在中性粒细胞的激活、迁移和趋化中起着关键作用。CD47在SU-DHL-2细胞中高表达。在图a中，使用DSPE-PEG-FITC标记的绿色荧光的Nm泡聚集在SU-DHL-2的细胞核周围，提示Nm泡可能粘附SU-DHL-2细胞并具有积极的靶向作用。

结论：FITC作为荧光标记部分，在特定波长的激发光下能够发出明亮的荧光，便于对标记的目标进行荧光检测和追踪，在生物成像细胞标记等众多生物领域有着应用前景。使用DSPE-PEG-FITC标记的绿色荧光的Nm泡聚集在SU-DHL-2的细胞核周围，提示Nm泡可能粘附SU-DHL-2细胞并具有积极的靶向作用。